To explore the factors related to successful vaginal trial of labor after cesarean (TOLAC) of advanced women. Methods: According to TOLAC success or not, 200 advanced women with ≥35 years old who had been given cesarean section from January 2020 to January 2023 were divided into group A (women with TOLAC succeeded) and group B (women with TOLAC failure). The clinical data of the women were compared between the two groups. Univariate and multivariate Logistic regression analysis were conducted to analyze the related factors affecting TOLAC success of the women. The maternal and infant outcomes in the two groups were counted. Results: There were 155 (77.5%) women with TOLAC succeeded and 45 (22.5%) women with TOLAC failure. The age, the time since the last cesarean section, the prenatal body mass index value, and the amniotic fluid situation of the women, and the newborn body mass in group A were all significantly lower than those in group B, and the Bishop score of cervix at delivery of the women and the proportions of the women without anxiety disorder or depression in group A were all significantly higher than those in group B (all P＜0.05). Logistic regression analysis showed that the high preclinical cervical Bishop score of the women was the factor promoting their TOLAC success (P＜0.05), while the long time since the last cesarean section, and the amniotic fluid abnormality, the anxiety and depression status of the women were the risk factors affecting their TOLAC success (P＜0.05). The proportion of postpartum hemorrhage (3.9%) of the women in group A was significantly lower than that (26.7%) of the women in group B (P＜0.05). Conclusion: The time since the last cesarean section, the amniotic fluid situation, the cervical ripeness, and the psychological states of the women can all affect their success of TOLAC, so many aspects of which should be need to consider, targeted intervention should be conducted to improve the success rate of TOLAC and to reduce the postpartum hemorrhage of the women.