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| Correlation between the fluorescence intensity of nicotinamide adenine dinucleotide phosphate in mouse oocytes and the morphology of the oocytes |
| 1. The 900th Hospital of Joint Support Force of Chinese People’s Liberation Army, Fuzhou, Fujian Province, 350025; 2.Fuzong Medical College of Fujian Medical University, Fuzhou, Fujian Province |
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Abstract To study the correlation between the fluorescence intensity of nicotinamide adenine dinucleotide phosphate（NADP）in mouse oocytes and the morphology of the oocytes. Methods: 82 oocytes with meiosis II (M II) of the mice were obtained by controlled ovarian hyperstimulation. These oocytes were divided into group A (8 fragment oocytes) and group B (5 oocytes with abnormality polar body) according to the different abnormal morphology of these oocytes. 9 oocytes with normal morphology were selected in group C. The distribution of NADP fluorophores in the oocytes was observed by two photon confocal microscopy, and the NADP fluorescence intensity of the oocytes was compared among the three groups. Results: The NADP fluorescence intensity (225.04±7.23) in the oocytes of group A was significantly higher than that (189.50±30.66) in the oocytes of group C (P<0.05), and which in the oocytes of group B had no significant different from that in the oocytes of group A and group C (P>0.05). Conclusion: The spontaneous fluorescence of NADP in abnormal mouse oocytes containing fragments is enhanced, and the abnormal NADP fluorescence can reflect the abnormal state of mitochondria of the oocytes, and which can be one means to evaluate the quality of oocytes.
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