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| Comparison of the screening efficacy of the high risk HPV E6/E7mRNA and HPV DNA for the cervical lesions of patients |
| Zhoushan Maternal and Child Health Care Hospital, Zhoushan, Zhejiang Province, 316000 |
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Abstract To explore the screening efficacy of the high risk human papilloma virus (HPV) E6/E7mRNA and HPV DNA for the cervical lesions of patients. Methods: 100 patients with positive TCT result as atypical squamous cell of undetermined significant (ASCUS) or above in routine physical examination, or the patients with contact bleeding, irregular vaginal bleeding, or vaginal abnormal discharge from October 2020 to October 2021 were collected. The detections of HPV E6/E7mRNA and HPV DNA and the cervical biopsy under the guidance of colposcopy of these patients were performed at the same time, and biopsy histopathology was used as the gold standard of the diagnosis of the cervical lesions. The expression levels of E6/E7 mRNA and DNA of high-risk subtypes HPV were analyzed. The detection rates of the cytology and histology were compared between the two detections of E6/E7 mRNA and DNA of high-risk subtypes HPV. Results: Based on the result of cytological diagnosis, there was no significant differences in the positive rates of HPV E6/E7 mRNA and HPV DNA (P>0.05). Based on the result of pathology as the gold standard, the positive rate of the detections of HPV E6/E7 mRNA and HPV DNA of the patients with >grading 2 of cervical intraepithelial neoplasia (CIN) was significantly higher than that of the patients with chronic cervicitis group or with grading 1 of CIN. The positive rate of HPV DNA detection of the patients with chronic cervicitis was significantly higher than that of HPV E6/E7 mRNA detection. The overall positive rate of HPV DNA detection of the patients was also significantly higher than that of HPV E6/E7 mRNA detection (all P<0.05). The results of pathology as the gold standard, the specificity (84.2%), the positive predictive value (71.7%), and the coincidence rate (81.8%) of HPV E6/E7 mRNA detection for screening the cervical lesions were significantly higher than those (51.4%, 45.2%, and 62.1%) of HPV DNA detection (all P<0.05), and there were no significant differences in the sensitivity and the negative predictive value between the two detections of HPV E6/E7 mRNA and HPV DNA for screening the cervical lesions (P>0.05). Conclusion: HPV E6/E7 mRNA detection can improve the specificity and the positive predictive value for screening the cervical lesions of the patients, and which can guide the shunting management of the cervical lesions.
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