|
|
|
| Levels of high mobility group protein B1 and nucleotide-binding oligomerization domain-like receptor protein 3 of pregnant women with threatened abortion and their correlation with the vaginal microecology |
| Daxing District People's Hospital of Beijing, Beijing, 100000 |
|
|
|
|
Abstract To investigate the correlation between the vaginal microecology of pregnant women with threatened abortion and the levels of their serum high mobility group protein B1 (HMGB1) and nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3). Methods: From February 2021 to February 2022, 96 pregnant women with threatened abortion were selected in study group, and 90 healthy pregnant women were selected in control group. The expression levels of serum HMGB1, NLRP3, and tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), and interleukin-2 (IL-2) of the women in the two groups were detected by enzyme-linked immunosorbent assay (ELISA). Pearson method was used to analyze the correlation between the levels of the serum HMGB1 and NLRP3 of the women with threatened abortion and their vaginal microecology. Results: The relative abundances of bacteroidetes, proteobacteria, actinobacteria, prevotella, and gardnerella of the women in the study group were significantly higher than those of the women in the control group, and which of fusobacteria of the women in the study group was significantly lower. The levels of TNF-α and IL-2 of the women in the study group were significantly higher than those of the women in the study group, but the IL-4 level was significantly lower. The levels of HMGB1 (23.51±2.56μmol/L) and NLRP3 (132.35±20.12 pg/ml) of the women in the study group were significantly higher than those (18.24±2.16μmol/L and 105.86±19.23 pg/ml) of the women in the control group (all P＜0.05). Correlation analysis showed that in the study group, the serum HMGB1 level of the women was positively correlated with their serum NLRP3 level (r=0.581). The serum HMGB1 and NLRP3 levels of the women were positively correlated with their relative abundances of bacteroides, proteobacteria, actinobacteria, prevotella, and gardella, and which were negatively correlated with their relative abundance of fusobacteria (all P＜0.05). Conclusion: The levels of serum HMGB1 and NLRP3 of the pregnant women with threatened abortion are increased abnormally, and which are related to the vaginal microecology indexes of the women.
|
|
|
|
|
|
|
|
|
