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| Correlation between the levels of reactive oxygen species and superoxide dismutase in follicular fluid of patients with tubal factor infertility and their low quality of eggs |
| No. 990 Hospital of Joint Logistics Support Force, Zhumadian Henan Province, 463000 |
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Abstract To study the correlation between the levels of reactive oxygen species (ROS) and superoxide dismutase (SOD) in follicular fluid of patients with tubal factor infertility and their low quality of eggs. Methods: The clinical data of 85 patients with tubal factor infertility who had accepted ovulation induction therapy by long-acting regimen and in vitro fertilization-Embryo transfer (IVF-ET) from April 2019 to January 2022 were collected. According to the results of embryo culture and the critical value was identified by 35% of high-quality embryos, 44 patients with >35% of high-quality embryos was in group A and 41 patients with <35% of high-quality embryos was in group B. The follicular fluid of the patients in the two groups was collected for detecting the levels of ROS and SOD. Fasting venous blood of the patients was obtained to detect the levels of their serum follicle stimulating hormone, luteinizing hormone, estrogen, and testosterone. The eggs of the patients in the two groups were extracted by puncture under transvaginal ultrasound for calculating the number of eggs and for observing the maturation rate of eggs. The fertilization rate of the patients was observed after 12 hours of IVF-ET, and the high-quality embryo rate of the patients was calculated after 72 hours of IVF-ET. Pearson method was used to analyze the correlation between the ROS and SOD levels of the patients and their hormone levels and egg quality. Results: The ROS level in follicular fluid (0.81±0.25 ng/ml) of the patients in group A was significantly lower than that (6.97±1.72 ng/ml) of the patients in group B, and the SOD level in follicular fluid (1.14±0.28 IU/ml) of the patients in group A was significantly higher than that (5.34±1.92 IU/ml) of the patients in group B (P＜0.05). There were no significant differences in the levels of follicle-stimulating hormone and estrogen of the patients between the two groups (P＞0.05), while the levels of luteinizing hormone and testosterone of the patients in group A were significantly lower than those of the patients in group B (P＜0.05). There was no significant difference in the number of eggs obtained of the patients between the two groups (P＞0.05). The rates of egg maturation, the fertilization, and high-quality embryo of the patients in group A were significantly higher than those of the patients in group B (P＜0.05). Pearson correlation analysis method showed that the luteinizing hormone and testosterone levels of the patients were positive correlation with their ROS level, and were negative correlation with their SOD level. The rates of fertilization and high-quality embryo of the patients were negative correlation with their ROS level, while were positively correlated with their SOD level (all P＜0.05). Conclusion: Oxidative stress may be the cause of the low-quality egg of the patients with tubal factors infertility, and the levels of ROS and SOD in follicular fluid of the patients are related to their sex hormones levels, the fertilization rate, and the high-quality embryo rate.
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