To explore the effect of continuous suture and interrupted suture of perineal lateral incision of women, and to study their influence on the recovery of the incision of the women. Methods: 141 women with perineal lateral incision after natural delivery were selected as the research subjects and were divided into two groups by random number table method between March 2019 and March 2021. 71 women in group A were given continuous suture of perineal lateral incision, and 70 women in group B were given interrupted suture of perineal lateral incision. The women in the two groups were followed up for 3 months. The time differences of incision suture, healing, incision pain, and hospital stay of the women were compared between the two groups. The incision healing situations of the women in the two groups after 3 days of delivery were analyzed. The sexual function of the women in the two groups in the 3th month after delivery were evaluated by Female Sexual Function Index (FSFI), and the incidences of the adverse reactions in incision of the women during hospitalization and follow-up were compared between the two groups. Results: The perineal incision suture time (15.5±2.7 min), the healing time (5.9±2.0 d), the incision pain time (2.3±0.6 d), and the time of hospital stay (5.8±1.1 d)of the women in group A were all significantly shorter than those (20.1±2.6 min, 7.3±1.9 d, 3.0±0.7 d, and 6.8±1.6d)of the women in group B. The wound healing quality of the women in group A in the 3rd day after delivery was significantly better than that of the women in group B. The scores of FSFI of the women in group A in the postpartum 3rd month were significantly higher than those of the women in group B (all P＜0.05). There were no significant differences in the incidence of the line reaction in incision, and the scar tenderness and infection of the women between the two groups (P＞0.05). The incidence of subcutaneous scleroma (2.8%)of the women in group A was significantly lower than that (11.4%)of the women in group B (P＜0.05). Conclusion: The operation time of continuous suture of perineal lateral incision of the women is shorter than that of interrupted suture, which is beneficial to the incision healing and the maternal sexual function, with less adverse reactions after suture.