|
|
|
| Efficacy of quantitative detection of paired boxed gene 1 methylation for screening high-grade cervical precancerous lesions |
| Sanmenxia Hospital of Yellow River, Sanmenxia, Henan Province, 472000 |
|
|
|
|
Abstract To investigate the value of quantitative detection of paired boxed gene 1(PAX1) methylation for diagnosing high-grade cervical precancerous lesions. Methods: From June 2021 to June 2022, a total of 64 patients with high-risk subtype HPV infection were selected as the study subjects. All the patients had received pathological diagnosis, and according to the results of pathological diagnosis, these patients were divided 19 cases with normal cervix in group A, 21 cases with low-grade cervical precancerous lesions in group B, and 24 cases with high-grade cervical precancerous lesions in group C. The cervical tissue samples of the patients in the three groups were collected, and the methylation level of PAX1 gene in the cervical tissue samples of the patients was detected by pyrosequencing method. The positive rate of HPV infection, and the positive rate and the level of PAX1 methylation in cervical tissue from cervical scrape of the patients were compared among the three groups. Receiver operating characteristic curve (ROC) was used to analyze the value of the level of PAX1 methylation in cervical tissue of the patients for diagnosing their high-grade cervical precancerous lesions. Results: There was no significant difference in the quantitative values of high-risk HPV DNA (392.17±95.62 vs. 442.18±113.47 vs. 533.38±203.15) of the patients among the three groups (P=0.204). The positive rates of HPV and PAX1 methylation (36.8% and 0) of the patients in group A, those (66.7% and 9.5%) of the patients in group B, and those (91.7% and 62.5%) of the patients in had increased gradually (all P＜0.05). ROC curve analysis showed that the area under the curve, the sensitivity, and the specificity of PAX1 methylation level detection of the patients for diagnosing their high-grade cervical precancerous lesions were 0.785, 89.5%, and 80.5%, respectively. Conclusion: The methylation level of the women detected by quantitative ion of PAX1 methylation can effectively diagnose their high-grade cervical precancerous lesions.
|
|
|
|
|
|
|
|
|
