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Abstract To investigate the effects of microRNA(miR)-214 targeting twist related protein 1(TWIST1)on the migration, invasion and epithelial stromal transformation of endometrial carcinoma HEC-1A cells. Methods: After miR-214 mimics were transfected into HEC-1A cells, the expressions of miR-214 and TWIST1 mRNA in HEC-1A cells were detected by real-time fluorescence quantitative PCR. The relationship between target miR-214 and TWIST1 was detected by double luciferase reporter gene assay. HEC-1A cells that had been in vitro cultured were divided into blank group, miR-214 mimics group, pcDNA-TWIST1 group, and miR-214 mimics + pcDNA-TWIST1 group. Western blot was used to detect the protein expressions of TWIST1, E-cadherin, N-cadherin, and Vimentin of the cells in these groups. Scratch test was used to detect the migration of the cells, and Transwell cell was used to detect the invasion of the cells. Results: After transfection of miR-214 mimics, the expression level of miR-214 in HEC-1A cells had increased, while the expression level of TWIST1 mRNA had decreased, and miR-214 could directly targeted combine with TWIST1. Compared with those in the blank group, the protein expression levels of TWIST1, N-cadherin, and Vimentin, the rate of scratch healing, and the number of invasive cells in miR-214 mimics group were significantly lower, while the protein expression level of E-cadherin was higher significantly(P＜0.05). Moreover, the protein expression levels of TWIST1, N-cadherin, and Vimentin, the rate of scratch healing, and the number of invasive cells in pcDNA-TWIST1 group were higher significantly, while the protein expression level of E-cadherin were lower significantly(P＜0.05). Compared with those in the pcDNA-TWIST1 group, the protein expression levels of TWIST1, N-cadherin, and Vimentin, the rate of scratch healing, and the number of invasive cells in miR-214 mimics + pcDNA-TWIST1 group were lower significantly, while the protein expression level of E-cadherin was higher significantly(P＜0.05). Conclusion: MiR-214 can inhibit the migration, invasion, and epithelial-stromal transformation of endometrial carcinoma HEC-1A cells by targeted regulating TWIST1.
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