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Abstract To explore the potential mechanism of mulberry parasitic- teasel root for treating polycystic ovary syndrome (PCOS) by network pharmacology. Methods: The active components of mulberry parasitic- teasel root were retrieved in traditional Chinese medicine systematic pharmacological database platform (TCMSP), and all the targets of which were predicted. The genes were standardized by uniprot database. The PCOS related targets were obtained in the OMIM and Genecards databases. The drug and disease targets were merged to take the intersection and display as a Venn diagram. The network map of drugcomponent-disease-target was built by Cytoscape 3.7.2 software. The protein interaction PPI network map was built by STRING database, and the core target network map of drug-disease was also built by Cytoscape 3.7.2 software. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) of the shared targets were analyzed by DAVIDA database. Results: A total of 10 active ingredients of mulberry parasitic-teasel root were obtained after screening, 105 targets were predicted, and 2164 targets of PCOS were obtained. A total of 77 drug-disease targets were obtained by Venn diagrams, and of which, VEGFA, IL6, TP53, PTGS2, MAPK1, JUN, TNF, IL1B, EGF, EGFR, CCL2, and other targets played the key roles in the treatment of PCOS by mulberry parasiticteasel root. GO and KEGG enrichment analysis yielded 126 biological items and 17 signaling pathways, of which, GO biological items involved in the positive regulations of nitric oxide biosynthetic process, cell proliferation, the extracellular space, the receptor activities of enzyme binding, steroid binding, steroid hormone, and other biological processes. KEGG pathway enrichment analysis found that the target genes were mainly enriched in estrogen signaling pathway, HIF-1 signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, cancer signaling pathway, and other signaling pathways. Conclusion: The treatment of PCOS by mulberry parasitic- teasel root has the characteristics of multiple components, multiple targets, and multiple pathways, which provides a theoretical basis for further study the action mechanism.
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