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| Expression of long non-coding NTF3-5 in peripheral blood of patients with polycystic ovary syndrome and its correlation with the insulin resistance of the patients |
| 1. Henan Institute of Reproductive Health Science and Technology, National Health Commission Key Laboratory of Birth Defect Prevention, Zhengzhou, Henan province, 450000; 2. The Third People's Hospital of Henan Province |
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Abstract To detect the expression of circulating long non-coding NTF3-5 (Lnc-NTF3-5) in peripheral blood of patients with polycystic ovary syndrome (PCOS), and to study the correlation between the Lnc-NTF3-5 level of the patients and their insulin resistance (IR). Methods: 108 patients with PCOS were included in study group during December 2018 to December 2020, and 108 healthy women with the matching age who underwent physical examinations were included control group during the same period. Fasting venous blood of the patients in the two groups were collected. Real-time fluorescence quantification and enzyme-linked immunosorbent assay were used to detect the levels of Lnc-NTF3-5 and CRP of the patients in the two groups, respectively. The patients in the study group were also divided into group A (the patients with IR) and group B (the patients without IR) according to homeostatic model assessment (HOMA-IR) index of the patients. The Lnc-NTF3-5 expression level and the HOMA-IR value of the patients were compared among these groups. Pearson method was used to analyze the correlation between the Lnc-NTF3-5 level of the patients with PCOS and their CRP and sex hormone levels, and their HOMA-IR value. Results: The levels of Lnc-NTF3-5 and CRP of the patients in the study group were significantly higher than those of the patients in the control group, and the Lnc-NTF3-5 level and the HOMA-IR value of the patients in group A were significantly higher than those of the patients in group B (all P<0.05). The expression of Lnc-NTF3-5 of the patients in the study group was positively correlated with their levels of CRP, luteinizing hormone, testosterone, and estradiol, and their HOMA-IR value, but was negatively correlated with their follicle stimulating hormone level (all P<0.05). Conclusion: The expression of Lnc-NTF3-5 in peripheral blood of the patients with PCOS is up-regulated, which may play an important role in their IR by affecting the inflammatory response and the sex hormone endocrine of the patients. It provides the new ideas for studying the potential mechanism of IR and the identification of new therapeutic targets of the patients with PCOS.
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