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| Significance of circulating melanoma antigen gene-A3 and melanoma antigen-A6 expressions in patients with cervical cancer |
| Hongci Hospital of Tangshan, Hebei Province, 063000 |
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Abstract To detect the expression levels of melanoma antigen gene-A3 (MAGE-A3) and melanoma antigen gene-A6 (MAGE-A6) in the peripheral blood circulation of patients with cervical cancer, and to explain their clinical significance. Methods: The peripheral blood circulation of 85 women with cervical cancer (in observation group) and 85 healthy volunteers (in control group) were collected from June 2015 to June 2016, and the monocyte were separate from the peripheral blood circulation of these women. The expression levels of MAGE-A3 and MAGE-A6 mRNA in the peripheral blood circulation of the women in the two groups were detected by fluorescence quantitative PCR technology, and the correlation between which of the women with cervical cancer and their clinical parameters was analyzed. The patients with cervical cancer were followed up for 5 years, and their survival status was recorded. Kaplan-Meier method was used to analyze the correlation between the expression levels of MAGE-A3 and MAGE-A6 mRNA of the women with cervical cancer and their prognosis. Results: The relative expression levels of MAGE-A3 (1.63±0.34) and MAGE-A6 mRNA (1.79±0.52) of the women in the observation group were significantly higher than those (1.00±0.00 and 1.00±0.00) of the women in the control group (P<0.05). The expression level of MAGE-A3 mRNA of the women in the observation group was correlated with their clinicopathological grade and lymph node metastasis of cervical cancer (P<0.05), but which was not correlated with their age, menopausal status, and pathological type of the women (P>0.05). The MAGE-A6 mRNA expression level of the women in the observation group was correlated with their lymph node metastasis (P<0.05), but which was not correlated with their age, menopausal status, pathological type and pathological grade of cervical cancer (P>0.05). In the observation group, the 5-year survival rate of the women with high expression level of MAGE-A3 mRNA or with high expression level of MAGE-A6 mRNA (41.9% or 44.2%) was significantly lower than that (69.1% or 66.7%) of the women with low expression level of MAGE-A3 mRNA or with low expression level of MAGE-A6 mRNA (P=0.008 or 0.014). Conclusion: The expression levels of MAGE-A3 and MAGE-A6 mRNA in the peripheral blood of the women with cervical cancer are abnormal increased, and which are closely related to the occurrence and development of cervical cancer. The high expression levels of MAGE-A3 and MAGE-A6 mRNA are related to the poor prognosis of the women with cervical cancer, which means that the MAGE-A3 and MAGE-A6 mRNA levels may be used as the clinical treatment targets and prognostic indicators of cervical cancer.
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