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| Expression and significance of adipose cell fatty acid binding protein, silent information regulator 1, soluble vascular cell adhesion factor of pregnant women with intrahepatic cholestasis of pregnancy |
| 1. Huixian People's Hospital, Xinxiang, Henan Province, 453600; 2. Liyuan Hospital Affiliated to Tongji Medical College, Huazhong University of science and technology, Wuhan, Hubei Province |
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Abstract To analyze the expression and the clinical significance of adipose cell fatty acid binding protein (AFABP), silent information regulator 1 (sirt1), sVCAM-1 (soluble vascular cell adhesion factor) of pregnant women with intrahepatic cholestasis of pregnancy (ICP). Methods: 60 pregnant women with ICP were selected in study group and 50 healthy pregnant women were selected in control group from January 2020 to January 2021. Enzyme-linked immunoassay (ELISA) method was used to detect the levels of AFABP and sVCAM-1 of the women in the two groups, and the real-time PCR method was used to detect the expression of sirt1 of the women in the two groups. The expression changes of the women and the diagnostic value of the levels of AFABP, sirt1, and sVCAM-1 for ICP were explored. Results: The sirt1 expression (0.26±0.03) of the women in the study group was significantly lower than that (1.12±0.11) of the women in the control group. The expression levels of AFABP (38.56±3.86μg/L) and SVCAM-1 (859.67±86.92μg/L) of the women in the study group were significantly higher than those (21.05±2.11μg/L and 572.25±57.59μg/L) of the women in the control group. In the study group, the sirt1 level of the women had decreased with the severity of disease significantly, and their AFABP and SVCAM-1 levels had increased with the severity of disease significantly (all P＜0.05). Correlation analysis showed that the AFABP level of the women in the study group was negatively correlated with their sirt1 level (r=-0.621, P=0.001), and which was positively correlated with their sVCAM-1 level (r=0.640, P=0.001), and the sirt1 level of the women in the study group was negatively correlated with their sVCAM-1 level (r=-0.647, P=0.001). ROC analysis showed that the combined AFABP, sirt1, and sVCAM-1 levels for diagnosing ICP had higher value than that of AFABP level, sirt1 level, or sVCAM-1 level alone (P＜0.05). The area under the curve, the sensitivity, and the accuracy of the combined AFABP, sirt1, and sVCAM-1 levels for diagnosing ICP were 0.867, 87.9%, and 88.7%, respectively. Conclusion: The expression of sirt1 of the pregnant women with ICP decreases, but their expressions of AFABP and sVCAM-1 increase, and the change degrees of which increase with the severity of their ICP. So the levels of sirt1, AFABP, and sVCAM-1 can be used to evaluate and diagnose ICP of the pregnant women in clinic.
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