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| Expression levels of peroxisome proliferator activated receptor β, preadipocytokine-1, serum amyloid A1 in subcutaneous fat of pregnant women with gestational diabetes mellitus and their correlation with the insulin index of the women |
| Rizhao Maternal and Child Health Care Hospital, Shandong Province, 276800 |
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Abstract To analyze the expression levels of peroxisome proliferator activated receptor β (PPARβ), preadipocytokine-1 (Pref-1), serum amyloid A1 (SAA1) in subcutaneous fat of pregnant women with gestational diabetes mellitus (GDM), and to study their correlation with insulin resistance (IR) of these women. Methods: A total of 153 pregnant women who underwent antenatal examination and gave birth from May 2017 to May 2020 were selected as study subjects prospectively. According to the results of OGTT test, these women were divided into group A (47 cases with GDM), group B (39 cases with impaired glucose tolerance (IGT)), and group C (67 cases with normal glucose tolerance (NGT)). The glucose oxidase assay was used to detect the level of blood glucose, radio-immunoassay (RIA) was used to detect the level of insulin (FINS), and qRT-PCR were used to detect the expressions of PPARβ, Pref-1, and SAA1 in subcutaneous tissues of these women. Results: The blood glucose level of the women in group A at different time point was significantly higher than that of the women in group C (P＜0.05), and the blood glucose level at 1h, 2h and 3h after oral administration of glucose of the women in group B was significantly higher than that of the women in group C (P＜0.05). The values of homeostasis model assessment insulin resistance index (HOMA-IR), early insulin secretion index (△I30/△G3), islet β cell secretion index (HBCI), and the levels of PPARβ, Pref-1, and SAA1 in subcutaneous fat of the women had significant different among the three groups (P＜0.05). The value of HOMA-IR and the SAA1 level of the women in group A, in group B, and in group C had decreased gradually. The
values of △I30/△G3 and HBCI, and the levels of PPARβ and Pref-1 of the women in group A, in group B, and in group C had increased gradually (all P＜0.05). Pearson analysis showed that the levels of PPARβ and Pref-1 of the women were negatively correlated with their HOMA-IR value (r=-0.529, -0.459, P＜0.05), and the SAA1 level of the women was positively correlated with their HOMA-IR value (r=0.747, P＜0.05). The levels of PPARβ and Pref-1 of the women were positively correlated with their △I30/△G30 and HBCI values (r=0.705, 0.750, 0.794, 0.795, P＜0.05), and the SAA1 level of the women was negatively correlated with their △I30/△G30 and HBCI values (r=-0.321, -0.429, P＜0.05). Conclusion: The expressions of PPARβ and Pref-1 in the subcutaneous fat of the pregnant women with GDM decrease, while the expression of their SAA1 level increases, and which all may involve in the process of insulin resistance of these women.
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