To explore the current situation of medication safety awareness and the situation of drugs used of pregnant women. Methods: A total of 1814 pregnant women were selected as the investigation subjects from January 2020 to December 2020. The status of medication safety awareness and the situation of drugs used during pregnancy were investigated and analyzed by questionnaire survey. Results: Among the 1814 pregnant women surveyed, most of them were 25-35 years old, college/bachelor degree of educational level, no stable job, and during 28-32 gestational weeks. In the 8 cognition questions about medication safety, the acquisition of knowledge about the time of folic acid used (95.0%) and calcium used (88.0%), and what gestational weeks with the most influence on the fetus of the drugs using (85.0%) of the respondents were generally good. 62.0% of the women had thought that they should not take drugs when they were sick during pregnancy, 43.3% of the women had believed that Chinese medicine was safer than western medicine, 52.9% of the women had known that there were adverse reactions of drugs even with the correct usage and dosage, 14.9% of the women had insufficient known that the grading of medication safety from American drug and food administration (DFA), and 15.9% of the women had also insufficient known that whether the sleeping pills could be taken safely during the second month of pregnancy. Among these women, 50% glucose injection (91.3%) was the most frequently used drug for them, and these women had positive attitude towards safe drug used. Logistic multifactor regression analysis showed that college education level or above, and the medical profession of the pregnant women were the factors promoting their drug used safely. Conclusion: The acquisition of knowledge about safe drug used of the pregnant women is relatively general, but their attitude of safe drug used is relatively positive. It is suggested that relevant administrative departments should carry out targeted health education and strengthen the popularization of medical knowledge for improving the awareness and the behavior of safe drug used of the pregnant women.