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| Expressions of cyclooxygenase-2 and matrix metalloproteinase-14 of patients with endometriosis and their correlation with the serum CA125 level |
| Rugao People's Hospital, Nantong, Jiangsu Province, 226500 |
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Abstract To detect the expressions of cyclooxygenase-2 (COX-2) and matrix metalloproteinase-14 (MMP-14) in endometriosis tittuse of patients, and to explore the correlation between the levels of COX-2 and MMP-14 of patients and their serum carbohydrate antigen 125 (CA125) level. Methods: 115 patients with endometriosis who wanted laparoscopic surgery were selected in observation group from March 2017 to March 2020, and 50 patients who had accepted hysteromyoma and were confirmed no abnormality of endometrium by pathology were selected in control group during the same time. The mRNA levels of COX-2 and MMP-14 in endometrial tissue of the patients in the two groups were detected by real-time fluorescent quantitative PCR (qRT-PCR), and their protein expressions of COX-2 and MMP-14 were detected by immunohistochemistry. Pearson method was used to analyze the correlation between the expressions of COX-2 and MMP-14 of the patients and their CA125 level. The value of COX-2 and MMP-14 expressions for diagnosing endometriosis was analyzed. Results: The mRNA levels of COX-2 (1.45±0.26) and MMP-14 (1.53±0.25), and the serum CA125 level (36.47±9.87 IU/ mL) in the endometrial tissue of the patients in the observation group had increased significantly. The positive rates of protein levels of COX-2 and MMP-14 of the patients in the observation group was significantly higher than those of the patients in the control group (all P＜0.05). In observation group, the expressions of COX-2 and MMP-14 of the patients were positively correlated with their serum CA125 level (r=0.619,0.515, all P＜0.05). The area under the curve, the sensitivity, and the specificity of the combined Cox-2 and MMP-14 levels for predicting endometriosis were 0.913, 86.1%, and 86.0%, respectively. Conclusion: COX-2 and MMP-14 are highly expressed in the endometrial tissues of the patients with endometriosis, and which are positively correlated with the serum CA125 level of the patients. Combined detection of COX-2 and MMP-14 has certain value for early diagnosing endometriosis, and which is expected to become the molecular markers for diagnosing endometriosis.
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