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| The correlation between the level of oxygen active substances of sperm and their morphology and DNA fragments |
| Joint Logistic Support Force 904 Hospital, Wuxi, Jiangsu Province, 214000 |
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Abstract To explore the correlation between the level of sperm oxygen active substances (OAS) of the men with infertility and their sperm morphological morphology and sperm DNA fragments, so as to provide theoretical basis for the antioxidation treatment of these men. Methods：250 male semen samples from August 2018 to August 2020 were selected to detect their sperm morphology, sperm DNA integrity, and the level of OAS. According to sperm morphology, these sperms were divided into group A1 (sperm with normal morphology), group A2 (sperm with head deformity, group A3 (sperm with neck deformity, group A4 (sperm with tail deformity), and group A5 (sperm with mixed deformity). These sperms were also divided into group B1 (sperm with sperm DNA fragment index (DFI) <15%), group B2 (sperm with sperm DFI 15%-30%, and group B3 (sperm with sperm DFI >30%) according to the rate of DFI. The sperm OAS levels in different groups were analyzed and its correlation with male infertility was analyzed. Results: Among these 250 sperm samples, there were 60.4% samples with normal sperm morphology, and there were 39.6% samples with abnormal sperm morphology, which included sperm head deformity of 47.5%, sperm neck deformity of 35.4%, sperm tail deformity 13.1%, and sperm mixed deformity 4.0%. There were no significant difference in the OAS level of sperm (715.49±203.82 U/L vs. 711.39±206.24 U/L vs. 718.83±201.57 U/L vs. 721.31±200.67 U/L) among group A2, group A3, group A4, and group A5, but the OAS level of sperm in group A2, group A3, group A4, or group A5 was all significantly higher than that (462.76±148.76 U/L) in group A1 (P<0.05). The rates of sperm DFI in group B1, group B2, and group B3 were 55.6%, 32.4%, and 12.0%, respectively, which had significant different among these groups. The OAS level of sperm in group B3 (719.74±205.39 U/L), in group B2 (637.24±183.65 U/L), and in group B1 (457.68±151.46 U/L) had decreased gradually (all P<0.05). Pearson correlation analysis showed that normal sperm morphology rate of the men was negatively correlated with their sperm OAS level. The rate of sperm neck deformity, sperm neck deformity, and sperm tail deformity of the men were positively correlated with their sperm OAS level, and the sperm head deformity rate had the highest correlation with the sperm OAS level. The rate of sperm DFI was positively correlated with the sperm OAS level (all P<0.05). Conclusion: The abnormal morphology of sperm may be an important source of oxygen free radicals, and the increase of sperm DNA fragments will increase the ROS level of sperm, which may accelerate the process of germ cell apoptosis and lead to male infertility.
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