To analyze the relationship between CYP19A1 RS2899470 polymorphism of infertility patients with endometriosis and their assisted reproduction outcomes (EMs). Methods: A total of 104 patients with EMs were selected and were divided 51 patients complicated with infertility who undergone IVF-ET in observation group and 53 were patients without infertility in control group from January 2017 to January 2020. The general clinical data of the patients in the two groups were observed. The number of eggs harvested, the fertilization rate, the cleavage number, and the pregnancy outcomes of infertility patients with CYP19A1 RS2899470 polymorphism after IVF-ET were analyzed. Results: The proportion of the patients carrying TT gene or TG+TT gene in the observation group was significantly higher than that of the patients in the control group, and carrying TT gene and TG and TT gene of the patients were correlated with their infertility (P<0.05). In the observation group, there were no significant differences in number of eggs harvested and the cleavage rate between the patients carrying GG gene and the patients carrying TG+TT gene (P>0.05). In the observation group, the number of M Ⅱeggs, the fertilization rate, and the high-quality embryo rate of the patients carrying GG gene were significantly higher than those of the patients carrying TG and TT gene (P<0.05), but there was no significant difference in the ectopic pregnancy rate between the patients carrying GG gene and the patients carrying TG and TT gene (P>0.05). In the observation group, the planting rate and the pregnancy rate of the patients carrying GG gene were significantly higher than those of the patients carrying TG+TT gene, and the abortion rate of the patients carrying GG gene was significantly lower (P>0.05). Conclusion: The patients with EMs and carrying TG and TT genes may have an increased risk of infertility, and carrying TG and TT genes can reduce the incidence of pregnancy of these patients after IVF-ET, which is not conducive to the pregnancy outcomes after IVF-ET.