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| Expression of TGF-β-induced factor homeobox 2 in cervical cancer tissues and its effect on proliferation, migration ,and invasion of cervical cancer cells |
| 1. The Second Affiliated Hospital of Guilin Medical University, Gui Lin, Guangxi Zhuang Autonomous Region, 541100; 2. Wuxi People's Hospital Affiliated to Nanjing Medical University |
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Abstract To investigate the expression of TGF-β-induced factor homeobox 2 (TGIF2) in cervical cancer tissues, and to study its effect on the proliferation, migration, and invasion of cervical cancer cells. Methods: The cervical cancer tissues samples of 76 patients with cervical cancer who underwent surgical treatment and were pathologically confirmed were selected in observation group from July 2016 to July 2019, and 65 normal cervical tissue samples were selected in control group. HeLa cells were also selected and were divided into three groups: blank control group, negative control group, and TGIF2 inhibition group. The expression level of TGIF2 was detected by immunohistochemistry. The correlation between the expression level of TGIF2 of cells and the clinicopathological characteristics of the patients was analyzed by univariate analysis. qRT-PCR was used to detect the expression level of TGIF2 in the transfected cells. Detection of cell proliferation activity was conducted by MTT test. Detection of cell invasion and migration was conducted by by Transwell scratch experiments. And Western blot was used to detect the protein expressions of TGIF2, cyclin D1, and MMP-2. Results: The positive expression rate of TGIF2 (81.6%) in the observation group was significant higher than that (18.5%) in the control group, and the positive expression of TGIF2 was correlated with the degree of cancer differentiation (P=0.004) and FIGO stage of cancer (P=0.009), but was not correlated with the patient's age, pathological type, and lymph node metastasis. The expression level (0.46±0.06) of TGIF2 mRNA TGIF2 inhibition group was significant lower than that (1.00±0.13) in blank control group and that (1.02±0.14)
in negative control group (P<0.05). Inhibition of TGIF2 expression had decresed the proliferation activity, migration, and invasion of HeLa cells in each group. Conclusion: The expression of TGIF2 in cervical cancer tissues increases, and inhibition of TGIF2 expression can decrease the proliferation, migration, and invasion of cervical cancer cells.
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