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| Relationship between the expressions of sterol regulatory element binding protein-1 and peroxisome proliferator-activated receptor α of women with gestational diabetes mellitus and their insulin resistance |
| 1. Tunchang County People's Hospital, Hainan Province, 571600；2. The Second Affiliated Hospital of Hainan Medical University |
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Abstract To detect the expressions of sterol regulatory element binding protein-1 (SREBP-1) and peroxisome proliferator-activated receptor α (PPARα) in peripheral blood of pregnant women with gestational diabetes mellitus (GDM), and to explore the relationship between them and insulin resistance. Methods: 90 pregnant women with GDM were selected in study group and 90 normal pregnant women were selected in control group from February 2018 to June 2020. The mRNA levels of SREBP-1 and PPARα in peripheral blood of the women in the two groups were detected by real time fluorescent quantitative PCR (qRT-PCR), and the protein levels of SREBP-1 and PPARα in peripheral blood of the women in the two groups were detected by Western Blot. The levels of fasting insulin (FINS) and fasting blood glucose (FPG) were detected by automatic electrochemiluminescence immunoassay, and the insulin resistance index (HOMA-IR) was calculated. The correlation between mRNA and protein expression levels of SREBP-1 and PPARα in peripheral blood of the women and their HOMA-IR value was analyzed by Pearson method. Results: The mRNA and protein expression levels of SREBP-1, the levels of FINS and FPG, and HOMA-IR value of the women in the study group were significant higher than those of the women in the control group, but the mRNA and protein levels of PPARαof the women in the study group were significant lower (all P<0.05). The mRNA and protein levels of SREBP-1 of the women in the study group were positively correlated with their HOMA-IR value (P<0.05), and the mRNA and protein levels of PPARαof the women in the study group were negatively correlated with their HOMA-IR value (P<0.05). Conclusion: The SREBP-1 level in the peripheral blood of the women with GDM increases significantly, but the PPARαlevel decreases significantly, both of which are related to the insulin resistance of the women, and they maybe participate in the occurrence of GDM by regulating the insulin resistance of the women.
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