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Abstract To detect the effect of formononetin on the expression of microRNA 587 (miR-587) and zinc ring finger protein 3 (ZNRF3), and to investigate the effect of formononetin on the migration and invasion of cervical cancer cells (Hela) by regulating miR-587/ZNRF3 axis. Methods: HeLa cells were cultured in vitro and divided into control group, 10 μmol/L formononetin group (drug group), 10 μmol/L formononetin+NC group (drug+NC group), 10 μmol/L formononetin+miR-587 mimics group (drug + miR-587 mimics group). The invasion and migration of HeLa cells were detected by Transwell method. The expression of ZNRF3, and invasion and migration related proteins were detected by Western blot. Double luciferase reporter genes were used to detect the targeting relationship between miR-587 and ZNRF3. qRT-PCR was used to detect the expression of miR-587 and ZNRF3 in the four groups. Results: Compared with those in the control group, the invasion and migration numbers of HeLa cells, the expression levels of MMP9, MMP-2, and miR-587 in the drug group were significant lower, while the expression level of ZNRF3 in the drug group was significant higher (P<0.05). Compared with those in the drug group, the invasion and migration numbers of HeLa cells and the expression levels of MMP9, MMP-2, miR-587, and ZNRF3 in the drug + NC group had no significant different (P>0.05). Compared with those in the drug group and the drug + NC group, the invasion and migration numbers of HeLa cells and the expression levels of MMP9, MMP-2, and miR-587 in the drug + miR-587 mimics group were significant higher, however which in the drug + miR-587 mimics group were still significant lower than those of the control group. The expression level of ZNRF3 in the drug + miR-587 mimics group was significant lower than those in in the drug group and the drug + NC group, but which was still significant higher than that in the control group (P<0.05). The results of target gene prediction showed that ZNRF3 was a potential target gene of miR-587, and miR-587 significantly down-regulated the expression of ZNRF3 (P<0.05). Conclusion: Formononetin can inhibit the migration and invasion of HeLa cells by inhibiting the expression of miR-587 and promoting the expression of ZNRF3.
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