To study the application effect of color Doppler ultrasound combined with magnetic resonance imaging (MRI) for diagnosing fetal gallbladder abnormalities. Methods: The clinical data of 5289 pregnant women were collected retrospectively. 5342 fetus of these women were given examined by color Doppler ultrasound and MRI. The efficacy of ultrasonography alone for diagnosing fetal gallbladder abnormalities compared with that of ultrasonography combined with MRI. Results: The display rate of fetal gallbladder and the detection rate of gallbladder abnormalities by prenatal ultrasound were 99.9% (5334/5342) and 0.7% (38/5342), which included 22 fetuses with gallbladder enlargement, 4 fetuses with small gallbladder, 3 fetuses with abnormal echo, and 1 fetus with double gallbladder. The display rate of fetal gallbladder and the abnormal gallbladder detection rate by MRI were 100.0% and 0.6% (33/5342), which included 3 fetuses with double gallbladder, 24 fetuses with gallbladder enlargement, and 6 fetuses with small gallbladder. With gestational weeks increasing, the length, the width, and the volume of the fetal gallbladder had increased by MRI (P＜0.05). The length, the width, the transverse diameter, and the volume of the fetal gallbladder during 32-35 gestational weeks were significant higher than those of the fetal gallbladder during 28-1 gestational weeks (P＜0.05). The false positive rate (0.02%) and false negative rate (8.57%) of prenatal ultrasound combined with MRI for diagnosing fetal gallbladder abnormalities were significant lower than those (0.35% and 14.29%) of ultrasound, and the positive predictive value (96.97%) of prenatal ultrasound combined with MRI for diagnosing fetal gallbladder abnormalities was significant higher than that (78.95%) of ultrasound (P＜0.05). Conclusion: the color ultrasound combined with MRI for diagnosing fetal gallbladder abnormalities have their own advantages and disadvantages. The combination of the color ultrasound and MRI can effectively reduce the risk of misdiagnosis of fetal gallbladder abnormalities.