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Abstract To identify differential proteins expression and to study its corresponded pathways involved in the occurrences and development of glioblastoma (GBM) of female patients in different age groups by proteomic analysis of lesion after biopsies. Methods: A total of 18 female patients with GBM admitted to Guangdong Sanjiu brain hospital were selected as the research objects. The patients were divided into group A (patients aged <50 years old) and group B (patients aged ≥50 years old). Data independent acquisition (DIA) proteomics analysis was performed to study high-throughput proteomics of these 18 female patients, and the differentially expressed proteins in the tumor tissues of female patients with GBM in different age groups were screened. Functional enrichment analysis and protein interaction network analysis were performed. Results: A total of 4617 proteins in the tumor tissues of these 18 female patients with GBM were identified, of which 3530 proteins were expressed in the tumor tissues of the patients in both groups. 99 proteins were differentially expressed in the tumor tissues of the patients in both groups by T test (P<0.05). The signaling pathways enrichment analysis showed that the proteins involved in the regulation of apoptosis and actin cytoskeleton of the patients in group B were up-regulated. The protein interaction networks analysis found that three potential key proteins were discovered to potentially regulate the progression of malignant GBM of the patients. Conclusion: Proteins differentially expresses in different age female patients with GBM. Alpha-1-acid glycoprotein 1, Annexin and Alpha-1-acid glycoprotein 2 may act as the key proteins to regulate the malignant proliferation of elderly female patients with GBM.
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