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| Effect and mechanism of BSYQHX prescription on ovarian reserve function of rats |
| 1.Hubei Maternal and Child Health Care Hospital, Wuhan, Hubei Province, 430070; 2.Hubei University of Traditional Chinese Medicine |
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Abstract To observe the effect and mechanism of BSYQHX prescription on ovarian reserve function of model rats with declined ovarian reserve (DOR). Methods: The DOR model rats were developed, and they were randomly divided into six groups (8 rats in each group). The rats in group A were given normal 15 ml/kg, the rats in group B were given tripterygium glycosides 50 mg/kg, the rats in group C were given estradiol valerate 0.2mg/kg, and the rats in group D1 were given BSYQHX prescription 4.31g/kg, the rats in group D2 were given BSYQHX prescription 8.62g/kg, and the rats in group D3 were given BSYQHX prescription 17.25g/kg. The vaginal smear in estrous cycle and the change of ovarian histomorphology of the rats in the 6 groups were observed under microscope. The follicle stimulating hormone (FSH) , luteinizing hormone (LH), levadiol (E2), and anti mullerian hormone (AMH) of the rats in the 6 groups were measured by ELLels, the expression of VEG and its receptor protein levels of the rats in the 6 groups were measured by immunohistochemistry, and the expression of VEGFmRNA in ovarian tissue of the rats in the 6 groups was detected by RT-PCR. Results: The duration of estrus cycle of the rats in group B had prolonged significantly, and the levels of ovarian and uterine indexes of the rats in group B had decreased significantly (P＜0.01). The serum levels of FSH and FSH/LH value of the rats in group B had increased significantly (P＜0.01), but the levels of AMH and E2 of the rats in group B had decreased significantly (P＜0.01). The levels of the ovarian and uterine indexes of the rats in group D3 were significant higher than those of the rats in group in group B (P＜0.01). The FSH level and FSH/LH value of the rats in group D2 and group D3 had been decreased significantly, and the E2 and AMH levels had been increased significantly (P＜0.01). The level of FSH of the rats in group D2 and group D3 was significant lower than that of the rats in group C group (P＜0.01). The level of E2 in D1 and D2 groups was significant higher than that of the rats in C group (P＜0.01). The levels of VEGF, its receptor Flt-1, and KDR protein of the rats in group B were significant lower than those of the rats in group A (P＜0.05). The expression levels of Flt-1 and KDR protein levels and their corresponding mRNA expressions of the rats in groups D1, D2 and D3 were significant higher than those of the rats in group B (P＜0.01), but which had no statistical significant different from those of the rats in group C (P>0.05). Conclusion: BSYQHX prescription can effectively improve the DOR of rats, reduce their FSH serum level and FSH/LH value, increase the levels of their E2 and AMH, and promote the neovascularization of their ovarian tissue. The mechanism may be related to the up regulation of VEGF/VEGFR protein and related to its corresponding mRNA expression.
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