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| Effects of oil-free condoms on sperm fertilization function and DFI |
| Zhongshan City People’s Hospital, Guangdong Province, 528400 |
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Abstract To observe the effect of oil-free condom on sperm fertilization function and the detection results of DNA fragment index (DFI) of sperm. Methods: 1.0ml liquefied semen was puted in the oil-free condom in the experimental group, which was placed in an oscillator and gently oscillated for 5min before being removed, and then was transferred to a 5ml centrifugation tube. 1.0ml liquefied semen was not in oil-free condom in the control group. Sperm motility in both groups was detected 15 min later, and then sperm acrosin activity, acrosomal reaction rate and DFI were immediately detected. The semen was compared between the experimental group and the control group. Results: The sperm motility of the experimental group was 58.7±12.4 % was significant lower than that (63.6±11.4 %) of the control group (P＜0.05). The activity of sperm acrosin of the experimental group was 102.0±26.3 U/106 sperm was significant higher than that (84.6±23.0 U/106 sperm ) of the control group (P＜0.05). The acrosomal reaction rate of the experimental group was 40.7±14.8 % was significant lower than that (48.4±15.8%) of the control group (P＜0.01). There was no significant difference in DFI between the two groups (P＞0.05). Conclusion: It is not recommended to use oilfree condoms to collect samples for the detection of sperm motility, sperm acrosin activity and sperm acrosome reaction.
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