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| The influence of cardamonin on motion parameters of human sperm suffering freezing thawing process |
| Fujian Maternity and Child Health Care Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian Province, 350001 |
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Abstract To evaluate the protective effects of cardamonin (CAR) on injury of human sperm that suffered freezing-thawing process, and to explore the development and improvement of novel sperm cryoprotectant. Methods: 25 semen samples were collected from healthy men and were pretreated in vitro before cryopreservation. The ratio of sperm suspension volume to cryopreservation agent volume was 1:1. The semen samples was divided into 6 groups, which included pre-cryopreservation group, freeze-thaw group, cryopreservation group, and protectant group with low concentration of CAR (0.5×10-6mol/L), protectant group with medium concentration of CAR (1.0×10-6 mol/L), and protectant group with high concentration of CAR (2.0×10-6 mol/L). The percentage of forward motility sperm (PR), the percentage of sperm overall motility (Motility), curvilinear velocity (VCL), velocity of straight line (VSL), average path velocity (VAP), mean angular displacement (MAD) and amplitude of lateral head displacement (ALH) were compared among the six groups. Results: Compared with those of the before cryopreservation group, the difference of all parameters of the cryopreservation group including PR, Motility, VCL, VAP, MAD and ALH had statistically significant different (P<0.05). The parameters including PR, Motility and VSL of semen in the cryoprotectant group were all statistical higher than those of semen in the cryopreservation group (P<0.05). The parameters of semen in the cryoprotectant group had significant different to those of semen in the protectant group with low, medium, or high concentration of CAR (F=5.07, P=0.030), and the PR of semen in the protectant group with high concentration of CAR was 47.2±0.6%, which was significant higher that (41.7±1.7%) of semen in the cryoprotectant group (LSD t=5.47, P=0.005). Conclusion: CAR combined with cryoprotectant can improve the forward motility of human sperm after suffered cryopreservation.
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