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| Release of Cu2+ in the uterus of rats by new ultrafine grained copper material and its effect on uterus tissue |
1. National Research Institute for Family Planning, Beijing, 100081; 2. Wu Jiang Yongyuan Biotech Co., Ltd.
3. Department of Materials Science and Engineering, College of Engineering, Peking University |
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Abstract Objective: To study the Cu2+ release of new ultrafine grained copper materials and ordinary copper materials in the uterine cavity, and to analyze its effect on the morphology of uterus of rats. Methods: Ordinary copper material (control group) and new ultra-fine crystal copper material (experimental group) were prepared as wires, and were implanted into the left uterus of 12-weeks-old female Wistar rats. And the right uterus of these rats hadn’t been implanted any material as selfcontrol. The rats were sacrificed at 12 h, or on the 1st d, 3rd d, 7th d, 15th d, and 30th d after the copper material implantated in the rat uterine cavity, and their peripheral serum, uterine lavage fluid, uterus and liver tissues were collected as samples. The concentration of Cu2+ in these samples was determined by inductively coupled plasma atomic emission spectrometry (ICP), and the histomorphology of the uterus tissue was analyzed. Results: There were no significant different in the concentration of Cu2+ in the serum and liver tissues of rats after implantation of copper material between the experimental group and the control group. The concentration of Cu2+ in the uterine lavage fluid of rats on the 3rd day in the two groups after implantation of copper material was the highest, and had decreased on 7th day and 15th day (P<0.05). The concentration of Cu2+ in the uterine lavage fluid of rats on the 5th or 7th day after implantation of copper material in the experimental group was significant lower than that of rats in the control group (P<0.05). The concentration of Cu2+ in the uterus of rats in the two groups at 12 h after implantation of copper material was the highest, and had decreased on the 1st day and the 3rd day (P<0.05), and then the concentration of Cu2+ in the uterus stabilized. The concentration of Cu2+ in the uterus of rats on the 7th or the 15th day of rats in the experimental group after implantation of copper material was significant lower than that of rats in the control group (P<0.05). The uterus tissue of rats in both groups showed acute aseptic inflammation early after implantation of copper material, but the tissue damage of rats in the experimental group showed a faster repaired. Conclusion: As a new material for intrauterine device, the new ultra-fine crystal copper material can significantly alleviate the phenomenon of Cu2+burst release, which may become an important alternative to existing copper materials.
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