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Abstract Objective: To investigate the changes of serum amino acid and acylcarnitine levels of women with gestational diabetes mellitus (GDM), and to explore their relationship with insulin resistance of women. Methods: 63 pregnant women with GDM were selected in the study group, and 58 normal pregnant women were included in the control group. The blood glucose levels of all included women were detected at 0h, 1h and 2h in the 75g oral glucose tolerance test (OGTT) during 24-28 gestational weeks. Serum insulin levels were determined by chemiluminescence method, and serum amino acid and acylcarnitine levels were determined by high performance liquid chromatography-tandem mass spectrometry. Results: The levels of fasting serum alanine (ALA), arginine (ARG), glycine (GLY), methionine (MET), acetyl carnitine (C2), Malonyl carnitine (C3DC)+C4OH, isovalerylcarnitine (C5DC)+C6OH, and hexanoyl meat the alkali(C6) of women in the study group were 266.2±53.6 umol/l, 21.1±5.6 umol/l, 171.6±40.1 umol/l, 13.6±2.8 umol/l, 7.68±2.44 umol/l, 0.05±0.02 umol/l, 0.06±0.01 umol/l, and 0.03±0.01 umol/l, respectively, which were significant higher than those (245.1± 43.7 umol/l, 18.5±5.8 umol/l, 156.7±32.9 umol/l, 11.9±2.5 umol/l, 5.84±1.76 umol/l, 0.04±0.01 umol/l, 0.05±0.01, and 0.02±0.01umol/l,vrespectively) of women in the control group (P<0.05 or 0.01). The levels of carnitine included free carnitine (C0) and Diacyl carnitine (C6DC) of women in the study group were 17.08±3.46 umol/l and 0.035±0.013 umol/l, which were significant lower than those (18.71±3.32umol/l and 0.044±0.015umol/l) of women in the control group (P<0.01). Pearson correlation analysis had showed that serum levels of ALA, ARG, MET, C2, C3DC+C4OH, C5DC+C6OH of women in study group were positive correlation to the levels of fasting insulin, 2h blood glucose, and insulin resistance index (HOMAIR) of women, while the levels of GLY and C6 of women were positively correlated to the 2h blood glucose level of women (P<0.05). Conclusion: The amino acid and acyl carnitine metabolism of women with GDM are abnormal, which may aggravate the degree of insulin resistance of women with GDM.
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