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| The reproductive inhibition effect of pcDNA3.1Crisp1 DNA vaccine on mice |
| 1.Zaozhuang Maternal and Child Health Care Hospital, Shandong Province, 277102；2. Anqiu Maternal and Child Health Care Hospital; 3. The Second Hospital Affiliated to Shandong University of Chinese Traditional Medicine |
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Abstract Objective: To investigate the reproductive inhibition effect of pcDNA3.1Crisp1 DNA vaccine. Methods: 20 female and 20 male SPF BALB/c mice aged 6-8 weeks were randomly divided into observation group A, observation group B, control group A, and control group B (10 mice in each group). The mice in the observation group A and B were muscle injected with recombinant plasmid pcDNA3.1 Crisp1, and the mice in the control group A and B were muscle injected with empty vector of pcDNA3.1 The serum Crisp 1 level of all mice were detected by ELISA. During the same time, the study of contraceptive effect was conducted in these normal mice, and their ovarian, testis and epididymis tissues were observed after HE staining. Results: The OD antibody levels of serum Crisp 1 of mice in the observation group A and B were (0.673±0.102) and (0.680±0.101), which were significant higher than those of mice in the control group A and B (P<0.05). The pregnancy rates of mice in the observation group A and B were 30.0% and 20.0%, which were significant lower than those of mice in the control group A and B (P<0.05). The number of baby mice in the observation group A and B was (5.6±0.3)pieces and (5.5±0.9)pieces, which were significant less than those of mice in the control group A and B (P<0.05). The ovarian tissues structures of all female mice were normal without atrophy. As for all male mice, the basement membrane of testis and epididymis were intact, and the diameter of seminiferous duct was normal, there were abundant sperm in testis and sperm cells, and there were plenty of mature sperm in epididymis. Conclusion: pcDNA3.1Crisp1 DNA vaccine has some inhibiting effect on fertility of mice, which should be researched further.
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