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Abstract To study the function of zinc finger protein ZNF222 in trophoblast cell proliferation, migration and invasion. Methods: Immunohistochemistry was used to detect the expression of ZNF222 in villiand decidua tissue of women with the first trimester of pregnancy and in endometrium tissue of non-pregnant women. After the knockdown of ZNF222 was done by small interfering RNA of human trophoblast cell, the cell proliferation was tested by CCK-8. The cell migration and invasion were tested by transwell. Results: ZNF222 expressed highly in villi tissue, but expressed weakly in decidua tissue of women with the first trimester of pregnancy, and ZNF222 expressed very weakly in the endometrial tissue of non-pregnant women. After the knockdown of ZNF222in human trophoblast cell line HTR8/SVneo, there was no significant change in cell proliferation (ZNF222 siRNA-1，P>0.05，P=0.610； ZNF222 siRNA-2，P>0.05，P=0.277), but cell migration and invasion had reduced significantly (migration: ZNF222 siRNA-1，P<0.05, P=0.0265，ZNF222 siRNA-2, P<0.01, P=0.00586; invasion: ZNF222 siRNA-1, P<0.05,P=0.0327，ZNF222 siRNA-2, P<0.05, P=0.0412). Conclusion: ZNF222 can regulate the migration and invasion of human trophoblast cells. It is speculated that ZNF222 may play an important role in human placenta formation.
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