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Abstract Objective: To investigate the molecular mechanism of expression of 11 beta HSD1 and 11 beta HSD2 in placental tissue of women with gestational diabetes mellitus (GDM). Methods: Normal placental tissue in vitro was treated by free fatty acids, dexamethasone, and insulin stimulation for 24h. The levels of gene and protein expression of 11 beta HSD1 and 11 beta HSD2 were detected by real time PCR and western blot method. Results: Compared with the BASAL condition, the levels of 11 beta HSD1 mRNA expression in the placenta tissues of PA group, DEX group, INS group, and MIX group were significantly lower (the level of each group was 1.00± 0.01, 0.54±0.03, 0.63 ± 0.10, 042 ± 0.04, or 0.61±0.07, respectively, P< 0.05), but the levels of 11 beta HSD2 mRNA expression in the placenta tissues of PA group, DEX group, INS group, and MIX group were significantly higher (the level of each group was 1.00±0.01, 1.46 ±0.12, 1.48±0.13, 1.53±0.08, and 1.93±0.09, respectively, P< 0.05) Compared with the BASAL condition, levels of 11 beta HSD1 protein expression in the placenta tissues of PA group, DEX group, INS group, and MIX group were significantly lower (the level of each group was 0.99 ± 0.01; 0.43±0.05, 0.18 ±0.02, 0.71 ± 0.09, and 0.54 ±0.06, respectively, P< 0.05), but the levels of 11 beta HSD2 protein expression were significantly higher (the level of each group was 1.04±0.04, 2.27±0.16, 4.10±0.98, 3.10±0.39, and 3.98±0.32, respectively, P<0.05). Conclusion: Hyperlipidemia, high cortisol or high insulin can be as single factor for regulating the expression of 11 beta HSDs in placental tissue. Hyperlipidemia, high cortisol or high insulin of women with GDM maybe the molecular mechanism of 11-beta HSDs regulation changes.
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