Objective: To compare the clinical outcome and application of subsequent frozen-thawed embryo transfer （FET） cycle and fresh embryo transfer (ET) cycle. Methods: The clinical pregnancy rate, implantation rate, live birth rate, ectopic pregnancy rate, miscarriage rate and ovarian hyper-stimulation syndrome (OHSS) rate were retrospectively analyzed between fresh ET（fresh ET group, n=324) and subsequent FET（FET group, n=179) after in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI)-ET from January 2011 to December 2013 in Center for Reproductive Medicine, Renmin Hospital of Wuhan University. Results: There were no significant differences in age, infertility duration, basal follicle-stimulating hormone (FSH), initial gonadotropin (Gn) dosage, days of stimulation, fertilization rate, 2PN rate, cleavage rate, good-quality embryo rate and numbers of embryo transferred between two groups (P all >0.05). The total Gn dosage in FET group was significantly lower than that in fresh ET group (P<005), but the levels of estradiol (E2) and progesterone (P) on human chorionic gonadotrophin (hCG) day, the number of follicle on hCG day and the number of oocyte retrieved were significantly higher than those in fresh ET group respectively (P<0.05). The clinical pregnancy rate (37.23%) and implantation rate (59.22%) in FET group were significantly higher than those in fresh ET group (29.56% vs. 49.07%, P＜0.05), while there were no significant differences in live birth rate, multiple pregnancy rate and ectopic pregnancy rate between two groups (P all >0.05), and FET group has relatively lower ovarian hyper-stimulation syndrome (OHSS) rate (0.56%) compared with fresh ET group. Conclusion: For patients at high risk of OHSS, freezing whole embryos and subsequent FET can significantly improve the clinical pregnancy rate and implantation rate, and it can also effectively prevent OHSS, and get satisfactory clinical outcome.